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Research article - Peer-reviewed, 2022

3D-Printed Fluorescence Hyperspectral Lidar for Monitoring Tagged Insects

Manefjord, Hampus; Muller, Lauro; Li, Meng; Salvador, Jacobo; Blomqvist, Sofia; Runemark, Anna; Kirkeby, Carsten; Ignell, Rickard; Bood, Joakim; Brydegaard, Mikkel;

Abstract

Insects play crucial roles in ecosystems, and how they disperse within their habitat has significant implications for our daily life. Examples include foraging ranges for pollinators, as well as the spread of disease vectors and pests. Despite technological advances with radio tags, isotopes, and genetic sequencing, insect dispersal and migration range remain challenging to study. The gold standard method of mark-recapture is tedious and inefficient. This paper demonstrates the construction of a compact, inexpensive hyperspectral fluorescence lidar. The system is based on off-the-shelf components and 3D printing. After evaluating the performance of the instrument in the laboratory, we demonstrate its efficient range-resolved fluorescence spectra in situ. We present daytime remote ranging and fluorescent identification of auto-powder-tagged honey bees. We also showcase range-, temporally- and spectrally-resolved free-flying mosquitoes, which were tagged through feeding on fluorescent-dyed sugar water. We conclude that violet light can efficiently excite administered sugar meals imbibed by flying insects. Our field experiences provide realistic expectations of signal-to-noise levels, which can be used in future studies. The technique is generally applicable and can efficiently monitor several tagged insect groups in parallel for comparative ecological analysis. This technique opens up a range of ecological experiments, which were previously unfeasible.

Keywords

Laser radar; Insects; Hyperspectral imaging; Fluorescence; Laser beams; Optical receivers; Instruments; Laser radar; fluorescence; hyperspectral sensors; remote sensing; environmental monitoring; instrumentation; ecology; pollination; disease vectors

Published in

IEEE Journal of Selected Topics in Quantum Electronics

2022, volume: 28, number: 5, article number: 7100109
Publisher: IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

Authors' information

Manefjord, Hampus
Lund University
Manefjord, Hampus
League of European Research Universities - LERU
Muller, Lauro
Lund University
Muller, Lauro
League of European Research Universities - LERU
Li, Meng
Lund University
Li, Meng
League of European Research Universities - LERU
Salvador, Jacobo
League of European Research Universities - LERU
Salvador, Jacobo
Lund University
Blomqvist, Sofia
Lund University
Blomqvist, Sofia
League of European Research Universities - LERU
Runemark, Anna
Lund University
Runemark, Anna
League of European Research Universities - LERU
Kirkeby, Carsten
University of Copenhagen
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Bood, Joakim
League of European Research Universities - LERU
Bood, Joakim
Lund University
Brydegaard, Mikkel
Lund University

UKÄ Subject classification

Ecology

Publication Identifiers

DOI: https://doi.org/10.1109/JSTQE.2022.3162417

URI (permanent link to this page)

https://res.slu.se/id/publ/116896