Skip to main content
Research article - Peer-reviewed, 2022

Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism

Miao, Rui; Siao, Wei; Zhang, Na; Lei, Zuliang; Lin, Deshu; Bhalerao, Rishikesh P.; Lu, Congming; Xu, Weifeng


Root hydrotropism refers to root directional growth toward soil moisture. Cortical microtubule arrays are essential for determining the growth axis of the elongating cells in plants. However, the role of microtubule reorganization in root hydrotropism remains elusive. Here, we demonstrate that the well-ordered microtubule arrays and the microtubule-severing protein KATANIN (KTN) play important roles in regulating root hydrotropism in Arabidopsis. We found that the root hydrotropic bending of the ktn1 mutant was severely attenuated but not root gravitropism. After hydrostimulation, cortical microtubule arrays in cells of the elongation zone of wild-type (WT) Col-0 roots were reoriented from transverse into an oblique array along the axis of cell elongation, whereas the microtubule arrays in the ktn1 mutant remained in disorder. Moreover, we revealed that abscisic acid (ABA) signaling enhanced the root hydrotropism of WT and partially rescued the oryzalin (a microtubule destabilizer) alterative root hydrotropism of WT but not ktn1 mutants. These results suggest that katanin-dependent microtubule ordering is required for root hydrotropism, which might work downstream of ABA signaling pathways for plant roots to search for water.


cortical microtubule arrays; oryzalin; KATANIN; abscisic acid; root hydrotropism

Published in

International Journal of Molecular Sciences
2022, volume: 23, number: 7, article number: 3846
Publisher: MDPI

Authors' information

Miao, Rui
Fujian Agriculture and Forestry University
Siao, Wei
Fujian Agriculture and Forestry University
Zhang, Na
Fujian Agriculture and Forestry University
Lei, Zuliang
Fujian Agriculture and Forestry University
Lin, Deshu
Fujian Agriculture and Forestry University
Beijing Forestry University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Lu, Congming
Shandong Agricultural University
Xu, Weifeng
Fujian Agriculture and Forestry University

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers


URI (permanent link to this page)