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Research article - Peer-reviewed, 2022

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Snell, Per; Wilkinson, Mark; Taylor, Gavin J.; Hall, Stephen; Sharma, Shrikant; Sirijovski, Nick; Hansson, Mats; Shewry, Peter R.; Hofvander, Per; Grimberg, Asa;

Abstract

Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of As WRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature As WRI1-wheat grains also had reduced weight, were wrinkled and had a shrunken endosperm and X-ray tomography revealed that the proportion of endosperm was decreased while that of the aleurone was increased. Grains were milled to produce two white flours and one bran fraction. Mineral and lipid analyses showed that the flour fractions from the As WRI1-wheat were contaminated with bran, due to the effects of the changed morphology on milling. This study gives a detailed analysis of grains from field grown transgenic wheat that expresses a gene that plays a central regulatory role in carbon allocation and significantly affects grain composition.

Keywords

aleurone cells; Triticum aestivum; carbon allocation; cereal endosperm; oil biosynthesis; transcription factor; triacylglycerol; wheat flour; WRINKLED1

Published in

Plants

2022, volume: 11, number: 7, article number: 889
Publisher: MDPI

Authors' information

Snell, Per
Swedish University of Agricultural Sciences, Department of Plant Breeding
Snell, Per
DLF Beet Seed AB
Wilkinson, Mark
Rothamsted research
Taylor, Gavin J.
Lund University
Hall, Stephen
Lund University
Hall, Stephen
Lund Inst Adv Neutron and Xray Sci LINXS
Swedish University of Agricultural Sciences, Department of Plant Breeding
Sirijovski, Nick
Lund University
Hansson, Mats
Lund University
Shewry, Peter R.
Rothamsted research
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Breeding

UKÄ Subject classification

Food Science

Publication Identifiers

DOI: https://doi.org/10.3390/plants11070889

URI (permanent link to this page)

https://res.slu.se/id/publ/116932