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Research article - Peer-reviewed, 2022

The female sex pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster

Borrero-Echeverry, Felipe; Solum, Marit; Trona, Federica; Becher, Paul G.; Wallin, Erika A.; Bengtsson, Marie; Witzgall, Peter; Lebreton, Sebastien;

Abstract

Specific mate communication and recognition underlies reproduction and hence speciation. Our study provides new insights in Drosophila melanogaster premating olfactory communication. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues. Female-produced, species specific volatile pheromone (Z)-4-undecenal (Z4-11Al) and male pheromone (Z)-11-octadecenyl acetate (cVA) interact with food odour in a sex-specific manner. Furthermore, Z4-11Al, which mediates upwind flight attraction in both sexes, also elicits courtship in experienced males. Two isoforms of the olfactory receptor Or69a are co-expressed in the same olfactory sensory neurons. Z4-11Al is perceived via Or69aB, while the food odorant (R)-linalool is a main ligand for the other variant, Or69aA. However, only Z4-11Al mediates courtship in experienced males, not (R)-linalool. Behavioural discrimination is reflected by calcium imaging of the antennal lobe, showing distinct glomerular activation patterns by these two compounds. Male sex pheromone cVA is known to affect male and female courtship at close range, but does not elicit upwind flight attraction as a single compound, in contrast to Z4-11Al. A blend of the food odour vinegar and cVA attracted females, while a blend of vinegar and female pheromone Z4-11Al attracted males, instead. Sex-specific upwind flight attraction to blends of food volatiles and male and female pheromone, respectively, adds a new element to Drosophila olfactory premating communication and is an unambiguous paradigm for identifying the behaviourally active components, towards a more complete concept of food-pheromone odour objects.

Keywords

Specific mate recognition; Olfaction; Neuroethology; ( Z )-11-Octadecenyl acetate; cis-Vaccenyl acetate; ( Z )-4-Undecenal

Published in

Journal of Insect Physiology

2022, volume: 137, article number: 104355
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

Authors' information

Borrero, Felipe
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Borrero, Felipe
Colombian Corporation for Agricultural Research
Solum, Marit
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Trona, Federica
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Wallin, Erika A.
Mid-Sweden University
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Lebreton, Sebastien
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Lebreton, Sebastien
IRSEA

UKÄ Subject classification

Zoology

Publication Identifiers

DOI: https://doi.org/10.1016/j.jinsphys.2022.104355

URI (permanent link to this page)

https://res.slu.se/id/publ/116947