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Research article - Peer-reviewed, 2022

Development and Metabolic Characterization of Horse Gram (Macrotyloma uniflorum Lam. (Verdc.)) Mutants for Powdery Mildew Resistance

Sudhagar, Rajaprakasam; Priyanka, Shanmugavel; Chockalingam, Vanniarajan; Sendhilvel, Vaithiyanathan; Souframanien, Jegadeesan; Raja, Kalimuthu; Kanagarajan, Selvaraju

Abstract

Horse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse gram mutants were screened for powdery mildew (PM) disease resistance using the grade 0 to 4. The mutants were grouped based on the disease level of 0 to 2 (resistant) and susceptible (3 to 4). The PM altered the chlorophyll fluorescence (a/b ratio), maturity duration, and yield attributing traits. The yield loss ranged from 4.55% to 72.66%. After affirming the resistance level, the resistant mutant (RM) with minimum yield loss (scale:0) and the susceptible mutant (SM) with maximum loss (scale:4) were used for metabolomic analysis through GC-MS. PM infection induced expression of 66 metabolites representing 32 functional classes. The number of unique classes in RM and SM was 13 and 11, respectively, while eight were common. A fold change in the common metabolites indicated an enhanced accumulation of amine, alcohol, and ester in RM. Along with pathogen-induced defensive metabolites, RM produced silane and fluorene, whose biological significance in disease resistance is unknown. Though SM expressed defence-related bio-molecules, it failed to yield better.

Keywords

E. polygoni; GC-MS; horse gram; metabolomics

Published in

Agronomy
2022, volume: 12, number: 4
Publisher: MDPI

Authors' information

Sudhagar, Rajaprakasam
Tamil Nadu Agricultural University
Priyanka, Shanmugavel
Tamil Nadu Agricultural University
Chockalingam, Vanniarajan
Tamil Nadu Agricultural University
Sendhilvel, Vaithiyanathan
Tamil Nadu Agricultural University
Souframanien, Jegadeesan
Bhabha Atomic Research Center (BARC)
Raja, Kalimuthu
Tamil Nadu Agricultural University
Swedish University of Agricultural Sciences, Department of Plant Breeding

UKÄ Subject classification

Genetics and Breeding
Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.3390/agronomy12040800

URI (permanent link to this page)

https://res.slu.se/id/publ/117022