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Research article - Peer-reviewed, 2022

LncRNA PMAT-PtoMYB46 module represses PtoMATE and PtoARF2 promoting Pb2+ uptake and plant growth in poplar

Chen, Panfei; Song, Yuepeng; Liu, Xin; Xiao, Liang; Bu, Chenhao; Liu, Peng; Zhao, Lei; Ingvarsson, Par K.; Wu, Harry X.; El-Kassaby, Yousry A.; Zhang, Deqiang

Abstract

Lead (Pb2+) is one of the most toxic heavy-metal contaminants. Fast-growing woody plants with substantial biomass are ideal for bioremediation. However, the transcriptional regulation of Pb2+ uptake in woody plants remains unclear. Here, we identified 226 Pb2+-induced, differentially expressed long non-coding RNAs (DELs) in Populus tomentosa. Functional annotation revealed that these DELs mainly regulate carbon metabolism, biosynthesis of secondary metabolites, energy metabolism, and signal transduction through their potential target genes. Association and epistasis analysis showed that the lncRNA PMAT (Pb2+-induced multidrug and toxic compound extrusion (MATE) antisense lncRNA) interacts epistatically with PtoMYB46 to regulate leaf dry weight, photosynthesis rate, and transketolase activity. Genetic transformation and molecular assays showed that PtoMYB46 reduces the expression of PtoMATE directly or indirectly through PMAT, thereby reducing the secretion of citric acid (CA) and ultimately promoting Pb2+ uptake. Meanwhile, PtoMYB46 targets auxin response factor 2 (ARF2) and reduces its expression, thus positively regulating plant growth. We concluded that the PMAT-PtoMYB46-PtoMATE-PtoARF2 regulatory module control Pb2+ tolerance, uptake, and plant growth. This study demonstrates the involvement of lncRNAs in response to Pb2+ in poplar, yielding new insight into the potential for developing genetically improved woody plant varieties for phytoremediating lead-contaminated soils.

Keywords

Lead; Populus; Long non-coding RNAs; Association genetics; Phytoremediation

Published in

Journal of Hazardous Materials
2022, volume: 433, article number: 128769
Publisher: ELSEVIER

Authors' information

Chen, Panfei
Beijing University of Agriculture
Chen, Panfei
Beijing Forestry University
Song, Yuepeng
Beijing Forestry University
Liu, Xin
Beijing Forestry University
Xiao, Liang
Beijing Forestry University
Bu, Chenhao
Beijing Forestry University
Liu, Peng
Beijing Forestry University
Zhao, Lei
Beijing Forestry University
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
El-Kassaby, Yousry A.
University of British Columbia
Zhang, Deqiang
Beijing Forestry University
Zhang, Deqiang
Beijing University of Agriculture

UKÄ Subject classification

Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1016/j.jhazmat.2022.128769

URI (permanent link to this page)

https://res.slu.se/id/publ/117245