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Research article - Peer-reviewed, 2022

Catching change in microbial diversity indicators under different soil organic matter managements: Higher taxonomic resolution, better discrimination?

Changey, Frederique; Nunan, Naoise; Herrmann, Anke M.; Lerch, Thomas Z.;

Abstract

Recent advances in molecular ecology have dramatically improved our knowledge of soil microbial diversity and offers new indicators of soil quality. The usefulness of diversity indices has never been greater as the astronomical amounts of data generated in the literature needs to be synthesized. Despite technical guidelines have been proposed to characterize soil microbiomes using high throughput sequencing, the effect of taxonomic resolution on diversity indices is still largely unknown. Here, we explored how downscaling to higher taxonomic resolution levels may affect alpha-and beta-diversity indices of bacterial communities exposed to different soil organic matter management. To this aim, we collected soil samples in a long-term experimental site (Ultuna, Sweden) where different mineral and organic fertilizers have been applied for since 1956. We used both massive amplicon sequencing from phylum to species (OTU) and molecular fingerprints (PLFA, DGGE and T-RFLP). Our results showed that the discrimination potential increased at finer taxonomic resolution for beta-diversity but not for alpha-diversity indices such as richness and evenness. Also, the relative importance of hierarchical drivers of soil microbial communities such as C, N and pH varied depending on the taxonomic resolution. This study also demonstrated that indicators generated by molecular fingerprints such as PLFA, DGGE and T-RFLP are still consistent to monitor the effect of agricultural management on beta-diversity but not on alpha-diversity, which is useful information as it allows for a better use of results in past literature. We encourage performing such comparative studies on wider surveys, including different contexts and other indicators, in order to increase the efficiency and the robustness of the use of sequencing data in soil biodiversity monitoring.

Keywords

Taxonomic resolution; Microbial communities; Diversity indices; Soil organic matter

Published in

Ecological Indicators

2022, volume: 139, article number: 108897
Publisher: ELSEVIER

Authors' information

Changey, Frederique
Universite Paris-Est-Creteil-Val-de-Marne (UPEC)
Changey, Frederique
CNRS - Institute of Chemistry (INC)
Swedish University of Agricultural Sciences, Department of Soil and Environment
The National Center for Scientific Research (CNRS)
Swedish University of Agricultural Sciences, Department of Soil and Environment
Lerch, Thomas Z.
Universite Paris-Est-Creteil-Val-de-Marne (UPEC)

Sustainable Development Goals

SDG15 Life on land

UKÄ Subject classification

Microbiology
Soil Science

Publication Identifiers

DOI: https://doi.org/10.1016/j.ecolind.2022.108897

URI (permanent link to this page)

https://res.slu.se/id/publ/117324