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Research article - Peer-reviewed, 2022

A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component

Leong, Jia Xuan; Raffeiner, Margot; Spinti, Daniela; Langin, Gautier; Franz-Wachtel, Mirita; Guzman, Andrew R.; Kim, Jung-Gun; Pandey, Pooja; Minina, Alyona E.; Macek, Boris; Hafren, Anders; Bozkurt, Tolga O.; Mudgett, Mary Beth; Boernke, Frederik; Hofius, Daniel; Uestuen, Suayib

Abstract

Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense-related autophagy in plant-bacteria interactions.

Keywords

autophagy; effectors; immunity; ubiquitination; xenophagy

Published in

EMBO Journal
2022, volume: 41, number: 13, article number: e110352
Publisher: WILEY

Authors' information

Leong, Jia Xuan
Eberhard Karls University of Tubingen
Raffeiner, Margot
Leibniz Institut fur Gemuse- und Zierpflanzenbau (IGZ)
Spinti, Daniela
Leibniz Institut fur Gemuse- und Zierpflanzenbau (IGZ)
Langin, Gautier
Eberhard Karls University of Tubingen
Franz-Wachtel, Mirita
Eberhard Karls University of Tubingen
Guzman, Andrew R.
Stanford University
Kim, Jung-Gun
Stanford University
Pandey, Pooja
Imperial College London
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Swedish University of Agricultural Sciences, Department of Plant Biology
Bozkurt, Tolga O.
Imperial College London
Mudgett, Mary Beth
Stanford University
Boernke, Frederik
Leibniz Institut fur Gemuse- und Zierpflanzenbau (IGZ)
Boernke, Frederik
University of Potsdam
Uestuen, Suayib
Eberhard Karls University of Tubingen
Uestuen, Suayib
Ruhr University Bochum

UKÄ Subject classification

Microbiology

Publication Identifiers

DOI: https://doi.org/10.15252/embj.2021110352

URI (permanent link to this page)

https://res.slu.se/id/publ/117407