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Research article - Peer-reviewed, 2022

Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana

Zhang, Ai; Matsuoka, Keita; Kareem, Abdul; Robert, Madalen; Roszak, Pawel; Blob, Bernhard; Bisht, Anchal; De Veylder, Lieven; Voiniciuc, Catalin; Asahina, Masashi; Melnyk, Charles W.

Abstract

Wound healing is a fundamental property of plants and animals that requires recognition of cellular damage to initiate regeneration. In plants, wounding activates a defense response via the production of jasmonic acid and a regeneration response via the hormone auxin and several ethylene response factor (ERF) and NAC domain-containing protein (ANAC) transcription factors. To better understand how plants recognize damage and initiate healing, we searched for factors upregulated during the horticulturally relevant process of plant grafting and found four related DNA binding with one finger (DOF) transcription factors, HIGH CAMBIAL ACTIVITY2 (HCA2), TARGET OF MONOPTEROS6 (TMO6), DOF2.1, and DOF6, whose expression rapidly activated at the Arabidopsis graft junction. Grafting or wounding a quadruple hca2, tmo6, dof2.1, dof6 mutant inhibited vascular and cell-wall-related gene expression. Furthermore, the quadruple dof mutant reduced callus formation, tissue attachment, vascular regeneration, and pectin methylesterification in response to wounding. We also found that activation of DOF gene expression after wounding required auxin, but hormone treatment alone was insufficient for their induction. However, modifying cell walls by enzymatic digestion of cellulose or pectin greatly enhanced TMO6 and HCA2 expression, whereas genetic modifications to the pectin or cellulose matrix using the PECTIN METHYLESTERASE INHIBITOR5 overexpression line or korrigan1 mutant altered TMO6 and HCA2 expression. Changes to the cellulose or pectin matrix were also sufficient to activate the wound-associated ERF115 and ANAC096 transcription factors, suggesting that cell-wall damage represents a common mechanism for wound perception and the promotion of tissue regeneration.

Published in

Current Biology
2022, volume: 32, number: 9, pages: 1883-1893
Publisher: CELL PRESS

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Biology
Matsuoka, Keita
Teikyo University
Valiya Kadavu, Abdul Kareem
Swedish University of Agricultural Sciences, Department of Plant Biology
Robert, Madalen
Leibniz Institut fur Pflanzenbiochemie
Roszak, Pawel
University of Cambridge
Blob, Bernhard
University of Cambridge
Bisht, Anchal
Ghent University
De Veylder, Lieven
Ghent University
Voiniciuc, Catalin
University of Florida
Asahina, Masashi
Teikyo University
Swedish University of Agricultural Sciences, Department of Plant Biology

UKÄ Subject classification

Cell and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.1016/j.cub.2022.02.069

URI (permanent link to this page)

https://res.slu.se/id/publ/117573