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Research article - Peer-reviewed, 2022

Basic Exploratory Study of Bisphenol A (BPA) Dietary Administration to Istrian Pramenka Rams and Male Toxicity Investigation

Sturm, Sabina; Weber, Klaus; Klinc, Primoz; Sporndly-Nees, Ellinor; Fakhrzadeh, Azadeh; Knific, Tanja; Skibin, Andrej; Fialova, Vera; Okazaki, Yoshimasa; Razinger, Tanja; Laufs, Jurgen; Kreutzer, Robert; Pogacnik, Milan; Svara, Tanja; Cerkvenik-Flajs, Vesna


Bisphenol A (BPA), an endocrine-disrupting chemical and environmental pollutant, has been reported by many researchers to induce male reproductive toxicity in different experimental models. In this study, we investigated whether long-term exposure for two months to 25 mu g/kg body weight (low dose) of BPA affects spermatogenesis or sperm quality in young Istrian Pramenka rams exposed via diet. We evaluated body and testicular weights, histopathology of testes and epididymides, and sperm analyses, and compared these parameters between the group of treated rams and the control group of rams. Although there were some differences between the two groups, these differences were not large or statistically significant. The only statistically significant difference was the lower epithelial height of seminiferous tubules in treated rams, compared to control rams. In addition to assessing toxicity, BPA concentrations in the blood plasma of treated rams were determined after the first administration, and the toxicokinetic parameters of total BPA were calculated. In this study, no major signs of altered reproduction in rams were detected.


bisphenol A; rams; toxicokinetics; male toxicity; sperm

Published in

2022, volume: 10, number: 5, article number: 224
Publisher: MDPI

Authors' information

Sturm, Sabina
University of Ljubljana
Weber, Klaus
AnaPath Serv GmbH
Klinc, Primoz
University of Ljubljana
Fakhrzadeh, Azadeh
Iranian Research Institute for Information Science and Technology (IRANDOC)
Knific, Tanja
University of Ljubljana
Skibin, Andrej
University of Ljubljana
Fialova, Vera
Biopharm, Research Institute of Biopharmacy and Veterinary Drugs
Okazaki, Yoshimasa
AnaPath Services
Razinger, Tanja
AnaPath Services
Laufs , Jurgen
AnaPath Services
Kreutzer, Robert
AnaPath Services
Pogacnik, Milan
University of Ljubljana
Svara, Tanja
University of Ljubljana
Cerkvenik-Flajs, Vesna
University of Ljubljana

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