Cell-specific activation of aflatoxin B-1 correlates with presence of some cytochrome P450 enzymes in olfactory and respiratory tissues in horse

Abstract

Horses may be exposed to aflatoxin B-1 (AFB(1)) via inhalation of mouldy dust, leading to high exposure of olfactory and respiratory tissues. In the present study the metabolic activation of AFB(1) was examined in olfactory and respiratory tissues in horse. The results showed covalent binding of AFB(1)-metabolites in sustentacular cells and cells of Bowman's glands in the olfactory mucosa, in some cells of the surface epithelium of nasal respiratory, tracheal, bronchial and bronchiolar mucosa and in some glands in these areas. Immunohistochemistry revealed that cells expressing proteins reacting with CYP 3A4- and CYP 2A6/2B6-antibodies had a similar distribution as those having capacity to activate AFB(1). Our data indicate that the cell-specific activation of AFB(1) correlates with presence of some CYP-enzymes in olfactory and respiratory tissues in horse. (C) 2003 Elsevier Science Ltd. All rights reserved.

Keywords

aflatoxin B1 cytochrome P450 enzymes olfactory and respiratory tissues

Published in

Research in Veterinary Science
2003, volume: 74, number: 3, pages: 227-233
Publisher: W B SAUNDERS CO LTD

SLU Authors

• Persson, Eva

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Tyden, Eva

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Larsson, Pia

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Tjälve, Hans

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Publication identifier

• DOI: https://doi.org/10.1016/S0034-5288(02)00191-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/1177