Abstract

In this study, we designed, developed, and optimized an enzymatic fiber degumming process. First, a Partially Purified Enzyme Solution (PPES) containing pectinase, cellulase and xylanase was co-produced via ultrasound-assisted fermentation of orange peels using Bacillus subtilis ABDR01. Then, degumming with PPES was optimized for pH, temperature, incubation time and PPES concentration. Finally, three sets of ultrasound-assisted degumming experiments - chemical, enzymatic, and coupled enzymatic-chemical degumming with reduced alkali concentration (0.5% NaOH, 30 min) were performed. Chemical degumming for 2 h with 1% NaOH at 98 degrees C resulted in residual gum content of 5.9%, whereas ultrasound-assisted (30 min, 25 kHz) chemical degumming reduced the gum content to 3.1%. At a pH of 7.5, temperature of 45 degrees C, 30 h incubation and 2% PPES, gum removal efficiency of >60% and fibers with <10% residual gum were produced. Coupling the ultrasound-assisted enzymatic degumming with reduced alkali chemical degumming resulted in gum content of <3%. (C) 2022 The Author(s). Published by Elsevier B.V.

Keywords

Degumming; Enzyme; Fiber strength; Process optimization; Textile processing; Ultrasonication

Published in

Environmental technology & innovation
2022, Volume: 28, article number: 102666
Publisher: ELSEVIER

SLU Authors

• Simha, Prithvi

  • Department of Energy and Technology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biocatalysis and Enzyme Technology


Publication identifier

DOI: https://doi.org/10.1016/j.eti.2022.102666

Permanent link to this page (URI)

https://res.slu.se/id/publ/118223