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Research article - Peer-reviewed, 2022

Coupling chemical degumming with enzymatic degumming of ultrasound pre-treated ramie fiber using Bacillus subtilis ABDR01

Yadav, Ashish; Simha, Prithvi; Sathe, Priyanka; Gantayet, Lalit Mohan; Pandit, Aniruddha


In this study, we designed, developed, and optimized an enzymatic fiber degumming process. First, a Partially Purified Enzyme Solution (PPES) containing pectinase, cellulase and xylanase was co-produced via ultrasound-assisted fermentation of orange peels using Bacillus subtilis ABDR01. Then, degumming with PPES was optimized for pH, temperature, incubation time and PPES concentration. Finally, three sets of ultrasound-assisted degumming experiments - chemical, enzymatic, and coupled enzymatic-chemical degumming with reduced alkali concentration (0.5% NaOH, 30 min) were performed. Chemical degumming for 2 h with 1% NaOH at 98 degrees C resulted in residual gum content of 5.9%, whereas ultrasound-assisted (30 min, 25 kHz) chemical degumming reduced the gum content to 3.1%. At a pH of 7.5, temperature of 45 degrees C, 30 h incubation and 2% PPES, gum removal efficiency of >60% and fibers with <10% residual gum were produced. Coupling the ultrasound-assisted enzymatic degumming with reduced alkali chemical degumming resulted in gum content of <3%. (C) 2022 The Author(s). Published by Elsevier B.V.


Degumming; Enzyme; Fiber strength; Process optimization; Textile processing; Ultrasonication

Published in

Environmental technology & innovation
2022, volume: 28, article number: 102666
Publisher: ELSEVIER

Authors' information

Yadav, Ashish
Institute of Chemical Technology - Mumbai
Swedish University of Agricultural Sciences, Department of Energy and Technology
Sathe, Priyanka
Institute of Chemical Technology - Mumbai
Gantayet, Lalit Mohan
Bhabha Atomic Research Center (BARC)
Pandit, Aniruddha
Institute of Chemical Technology - Mumbai

UKÄ Subject classification

Biocatalysis and Enzyme Technology

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