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Research article - Peer-reviewed, 2022

Genetic diversity of the pea root pathogen Aphanomyces euteiches in Europe

Kalin, Carol; Berlin, Anna; Brantestam, Agnese Kolodinska; Dubey, Mukesh; Arvidsson, Anna-Kerstin; Riesinger, Paul; Elfstrand, Malin; Karlsson, Magnus;

Abstract

The oomycete pathogen Aphanomyces euteiches causes root rot in various legume species. In this study we focused on A. euteiches causing root rot in pea (Pisum sativum), thereby being responsible for severe yield losses in pea production. We aimed to understand the genetic diversity of A. euteiches in Europe, covering a north-to-south gradient spanning from Sweden, Norway and Finland to the UK, France and Italy. A collection of 85 European A. euteiches strains was obtained, all isolated from infected pea roots from commercial vining pea cultivation fields. The strains were genotyped using 22 simple-sequence repeat markers. Multilocus genotypes were compiled and the genetic diversity between individual strains and population structure between countries was analysed. The population comprising strains from Italy was genetically different and did not share ancestry with any other population. Also, strains originating from Finland and the eastern parts of Sweden were found to be significantly different from the other populations, while strains from the rest of Europe were more closely related. A subset of 10 A. euteiches strains from four countries was further phenotyped on two susceptible pea genotypes, as well as on one genotype with partial resistance towards A. euteiches. All strains were pathogenic on all pea genotypes, but with varying levels of disease severity. No correlation between the genetic relatedness of strains and virulence levels was found. In summary, our study identified three genetically distinct groups of A. euteiches in Europe along a north-to-south gradient, indicating local pathogen differentiation.

Keywords

Aphanomyces root rot; genetic diversity; pathogenicity; Pisum sativum; virulence

Published in

Plant Pathology

2022, volume: 71, number: 7, pages: 1570-1578
Publisher: WILEY

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Kolodinska Brantestam, Agnese
Nomad Foods
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Arvidsson, Anna-Kerstin
Nomad Foods
Riesinger, Paul
Novia University of Applied Sciences
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology

UKÄ Subject classification

Genetics
Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1111/ppa.13598

URI (permanent link to this page)

https://res.slu.se/id/publ/118235