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Research article2021Peer reviewedOpen access

Determination of conifer age biomarker DAL1 interactome using Y2H-seq

Chen, Xi; Zhu, Qianya; Nie, Yumeng; Han, Fangxu; Li, Yue; Wu, Harry; Niu, Shihui


Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.


Ageing; Conifer; DAL1; Proteins-interaction; Y2H-seq

Published in

Forestry Research
2021, Volume: 1, article number: 12

    UKÄ Subject classification

    Developmental Biology
    Medical Biotechnology
    Bioinformatics and Systems Biology

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