Skip to main content
Research article - Peer-reviewed, 2022

Genetic and Molecular Characterization of a New EMS-Induced Mutant without the Third Glucose Moiety at the C-3 Sugar Chain of Saponin in Glycine max (L.) Merr.

Ko, Jeongyun; Sundaramoorthy, Jagadeesh; Tsukamoto, Chigen; Park, Junbeom; Lee, Jinwon; Jo, Hyun; Lee, Jeong-Dong; Seo, Hak Soo; Song, Jong Tae

Abstract

Saponin, a secondary metabolite, is produced by various plant species, including soybean (Glycine max (L.) Merr.). Soybeans synthesize triterpenoid saponins, which are classified by their aglycone structure and sugar chain composition. Here, we characterized an ethyl methanesulfonate-induced mutant, PE1539, without saponin and with a glucose moiety at the third position of the C-3 sugar chain. The saponin phenotype of PE1539 is described by the accumulation of Ab-gamma g saponin and deficiency of Ab-alpha g saponin and DDMP-alpha g saponin, similar to a previously reported sg-3 mutant in soybean. Genetic analysis showed that the saponin phenotype of PE1539 is controlled by a recessive mutation. We mapped the gene responsible for the phenotype of PE1539 and the mapped region included Sg-3 (Glyma.10G104700). Further analysis of Sg-3 in PE1539 using DNA sequencing revealed a single-nucleotide substitution in the exon (G804A), resulting in a premature stop codon; thus, PE1539 produced a PSPG box-truncated protein. Saponin phenotype analysis of the F-2 population-from a cross between wild-type Uram and PE1539-showed that the phenotype of saponin was cosegregated with the genotype of Sg 3. Quantitative real-time PCR showed reduced expression of Sg-3 in PE1539 cells. Together, our data indicate that the saponin phenotype of PE1539 results from a mutation in Sg-3.

Keywords

soybean; saponin; saponin phenotype; Sg-3; UDP-glycosyltransferase

Published in

Agronomy
2022, volume: 12, number: 7, article number: 1598
Publisher: MDPI

Authors' information

Ko, Jeongyun
Kyungpook National University
Swedish University of Agricultural Sciences, Department of Plant Breeding
Kyungpook National University
Tsukamoto, Chigen
Iwate University
Park, Junbeom
Kyungpook National University
Lee, Jinwon
Kyungpook National University
Jo, Hyun
Kyungpook National University
Lee, Jeong-Dong
Kyungpook National University
Seo, Hak Soo
Seoul National University (SNU)
Song, Jong Tae
Kyungpook National University

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.3390/agronomy12071598

URI (permanent link to this page)

https://res.slu.se/id/publ/118597