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Research article - Peer-reviewed, 2022

Metabolome and Transcriptome Analyses Unravels Molecular Mechanisms of Leaf Color Variation by Anthocyanidin Biosynthesis in Acer triflorum

Sun, Anran; Pei, Xiaona; Zhang, Shikai; Han, Zhiming; Xie, Ying; Qu, Guanzheng; Hu, Xiaoqing; Tigabu, Mulualem; Zhao, Xiyang

Abstract

Acer triflorum Komarov is an important ornamental tree, and its seasonal change in leaf color is the most striking feature. However, the quantifications of anthocyanin and the mechanisms of leaf color change in this species remain unknown. Here, the combined analysis of metabolome and transcriptome was performed on green, orange, and red leaves. In total, 27 anthocyanin metabolites were detected and cyanidin 3-O-arabinoside, pelargonidin 3-O-glucoside, and peonidin 3-O-gluside were significantly correlated with the color development. Several structural genes in the anthocyanin biosynthesis process, such as chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR), were highly expressed in red leaves compared to green leaves. Most regulators (MYB, bHLH, and other classes of transcription factors) were also upregulated in red and orange leaves. In addition, 14 AtrMYBs including AtrMYB68, AtrMYB74, and AtrMYB35 showed strong interactions with the genes involved in anthocyanin biosynthesis, and, thus, could be further considered the hub regulators. The findings will facilitate genetic modification or selection for further improvement in ornamental qualities of A. triflorum.

Keywords

anthocyanin; metabolomics; ornamental tree; structural genes; transcription factors; transcriptome

Published in

Horticulturae
2022, volume: 8, number: 7, article number: 635
Publisher: MDPI

Authors' information

Sun, Anran
Jilin Agricultural University
Sun, Anran
Northeast Forestry University - China
Pei, Xiaona
Jilin Agricultural University
Zhang, Shikai
Jilin Agricultural University
Zhang, Shikai
Northeast Forestry University - China
Han, Zhiming
Northeast Forestry University - China
Xie, Ying
Northeast Forestry University - China
Qu, Guanzheng
Northeast Forestry University - China
Hu, Xiaoqing
Jilin Agricultural University
Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre
Zhao, Xiyang
Jilin Agricultural University
Zhao, Xiyang
Northeast Forestry University - China

UKÄ Subject classification

Agricultural Science
Botany

Publication Identifiers

DOI: https://doi.org/10.3390/horticulturae8070635

URI (permanent link to this page)

https://res.slu.se/id/publ/118603