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Research article - Peer-reviewed, 2022

A seed-like proteome in oil-rich tubers

Niemeyer, Philipp William; Irisarri, Iker; Scholz, Patricia; Schmitt, Kerstin; Valerius, Oliver; Braus, Gerhard H.; Herrfurth, Cornelia; Feussner, Ivo; Sharma, Shrikant; Carlsson, Anders S.; de Vries, Jan; Hofvander, Per; Ischebeck, Till;

Abstract

There are numerous examples of plant organs or developmental stages that are desiccation-tolerant and can withstand extended periods of severe water loss. One prime example are seeds and pollen of many spermatophytes. However, in some plants, also vegetative organs can be desiccation-tolerant. One example are the tubers of yellow nutsedge (Cyperus esculentus), which also store large amounts of lipids similar to seeds. Interestingly, the closest known relative, purple nutsedge (Cyperus rotundus), generates tubers that do not accumulate oil and are not desiccation-tolerant. We generated nanoLC-MS/MS-based proteomes of yellow nutsedge in five replicates of four stages of tuber development and compared them to the proteomes of roots and leaves, yielding 2257 distinct protein groups. Our data reveal a striking upregulation of hallmark proteins of seeds in the tubers. A deeper comparison to the tuber proteome of the close relative purple nutsedge (C. rotundus) and a previously published proteome of Arabidopsis seeds and seedlings indicates that indeed a seed-like proteome was found in yellow but not purple nutsedge. This was further supported by an analysis of the proteome of a lipid droplet-enriched fraction of yellow nutsedge, which also displayed seed-like characteristics. One reason for the differences between the two nutsedge species might be the expression of certain transcription factors homologous to ABSCISIC ACID INSENSITIVE3, WRINKLED1, and LEAFY COTYLEDON1 that drive gene expression in Arabidopsis seed embryos.

Keywords

lipid droplets; yellow nutsedge; Cyperus esculentus; tubers; seeds; proteome; Cyperus rotundus; Arabidopsis thaliana

Published in

Plant Journal

2022, volume: 112, number: 2, pages: 518-534
Publisher: WILEY

Authors' information

Niemeyer, Philipp William
University of Gottingen
Irisarri, Iker
University of Gottingen
Scholz, Patricia
University of Gottingen
Schmitt, Kerstin
University of Gottingen
Valerius, Oliver
University of Gottingen
Braus, Gerhard H.
University of Gottingen
Herrfurth, Cornelia
University of Gottingen
Feussner, Ivo
University of Gottingen
Swedish University of Agricultural Sciences, Department of Plant Breeding
Carlsson, Anders S. (Carlsson, Anders)
Swedish University of Agricultural Sciences, Department of Plant Breeding
de Vries, Jan
University of Gottingen
Swedish University of Agricultural Sciences, Department of Plant Breeding
Ischebeck, Till
University of Gottingen
Ischebeck, Till
University of Munster

UKÄ Subject classification

Soil Science
Botany

Publication Identifiers

DOI: https://doi.org/10.1111/tpj.15964

URI (permanent link to this page)

https://res.slu.se/id/publ/119072