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Research article - Peer-reviewed, 2022

Efficient carbon and nitrogen transfer from marine diatom aggregates to colonizing bacterial groups

Arandia-Gorostidi, Nestor; Berthelot, Hugo; Calabrese, Federica; Stryhanyuk, Hryhoriy; Klawonn, Isabell; Iversen, Morten; Nahar, Nurun; Grossart, Hans-Peter; Ploug, Helle; Musat, Niculina;

Abstract

Bacterial degradation of sinking diatom aggregates is key for the availability of organic matter in the deep-ocean. Yet, little is known about the impact of aggregate colonization by different bacterial taxa on organic carbon and nutrient cycling within aggregates. Here, we tracked the carbon (C) and nitrogen (N) transfer from the diatom Leptocylindrus danicus to different environmental bacterial groups using a combination of C-13 and N-15 isotope incubation (incubated for 72 h), CARD-FISH and nanoSIMS single-cell analysis. Pseudoalteromonas bacterial group was the first colonizing diatom-aggregates, succeeded by the Alteromonas group. Within aggregates, diatom-attached bacteria were considerably more enriched in C-13 and N-15 than non-attached bacteria. Isotopic mass balance budget indicates that both groups showed comparable levels of diatom C in their biomass, accounting for 19 +/- 7% and 15 +/- 11%, respectively. In contrast to C, bacteria of the Alteromonas groups showed significantly higher levels of N derived from diatoms (77 +/- 28%) than Pseudoalteromonas (47 +/- 17%), suggesting a competitive advantage for Alteromonas in the N-limiting environments of the deep-sea. Our results imply that bacterial succession within diatom aggregates may largely impact taxa-specific C and N uptake, which may have important consequences for the quantity and quality of organic matter exported to the deep ocean.

Published in

Scientific Reports

2022, volume: 12, number: 1, article number: 14949
Publisher: NATURE PORTFOLIO

Authors' information

Arandia-Gorostidi, Nestor
Helmholtz Center for Environmental Research (UFZ)
Arandia-Gorostidi, Nestor
Stanford University
Arandia-Gorostidi, Nestor
University of Gothenburg
Berthelot, Hugo
Helmholtz Center for Environmental Research (UFZ)
Berthelot, Hugo
Ifremer
Calabrese, Federica
Helmholtz Association
Calabrese, Federica
Harvard University
Stryhanyuk, Hryhoriy
Helmholtz Association
Klawonn, Isabell
Stockholm University
Klawonn, Isabell
Leibniz Institut fur Ostseeforschung Warnemunde
Iversen, Morten
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research
Iversen, Morten
University of Bremen
Nahar, Nurun
Swedish University of Agricultural Sciences, Department of Plant Biology
Nahar, Nurun
University of Gothenburg
Grossart, Hans-Peter
University of Potsdam
Grossart, Hans-Peter
Leibniz Institut fur Gewasserokologie und Binnenfischerei (IGB)
Ploug, Helle
University of Gothenburg
Musat, Niculina
Helmholtz Association

UKÄ Subject classification

Microbiology
Ecology

Publication Identifiers

DOI: https://doi.org/10.1038/s41598-022-18915-0

URI (permanent link to this page)

https://res.slu.se/id/publ/119080