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Research article - Peer-reviewed, 2022

Bacterial composition in Swedish raw drinking water reveals three major interacting ubiquitous metacommunities

Brindefalk, Bjorn; Brolin, Harald; Save-Soderbergh, Melle; Karlsson, Edvin; Sundell, David; Wikstrom, Per; Jacobsson, Karin; Toljander, Jonas; Stenberg, Per; Sjodin, Andreas; Dryselius, Rikard; Forsman, Mats; Ahlinder, Jon;

Abstract

Background Surface raw water used as a source for drinking water production is a critical resource, sensitive to contamination. We conducted a study on Swedish raw water sources, aiming to identify mutually co-occurring metacommunities of bacteria, and environmental factors driving such patterns. Methods The water sources were different regarding nutrient composition, water quality, and climate characteristics, and displayed various degrees of anthropogenic impact. Water inlet samples were collected at six drinking water treatment plants over 3 years, totaling 230 samples. The bacterial communities of DNA sequenced samples (n = 175), obtained by 16S metabarcoding, were analyzed using a joint model for taxa abundance. Results Two major groups of well-defined metacommunities of microorganisms were identified, in addition to a third, less distinct, and taxonomically more diverse group. These three metacommunities showed various associations to the measured environmental data. Predictions for the well-defined metacommunities revealed differing sets of favored metabolic pathways and life strategies. In one community, taxa with methanogenic metabolism were common, while a second community was dominated by taxa with carbohydrate and lipid-focused metabolism. Conclusion The identification of ubiquitous persistent co-occurring bacterial metacommunities in freshwater habitats could potentially facilitate microbial source tracking analysis of contamination issues in freshwater sources.

Keywords

16S rRNA; anthropogenic effects; bacterial community analysis; biotic interactions; generalized linear latent variable model

Published in

Microbiology Open

2022, volume: 11, number: 5, article number: e1320
Publisher: WILEY

Authors' information

Brindefalk, Bjorn
FOI - Swedish Defence Research Agency
Brolin, Harald
University of Gothenburg
Säve‐Söderbergh, Melle
Swedish National Food Agency
Säve‐Söderbergh, Melle
Karolinska University Hospital
Karlsson, Edvin
Swedish Defence Research Agency (FOI)
Karlsson, Edvin
Umea University
Sundell, David
FOI - Swedish Defence Research Agency
Wikström, Per
Swedish Defence Research Agency (FOI)
Toljander, Jonas
Swedish Defence Research Agency (FOI)
Stenberg, Per
FOI - Swedish Defence Research Agency
Stenberg, Per
Umea University
Sjodin, Andreas
FOI - Swedish Defence Research Agency
Dryselius, Rikard
Swedish National Food Agency
Forsman, Mats
FOI - Swedish Defence Research Agency
Ahlinder, Jon
FOI - Swedish Defence Research Agency

Sustainable Development Goals

SDG6 Clean water

UKÄ Subject classification

Microbiology
Oceanography, Hydrology, Water Resources

Publication Identifiers

DOI: https://doi.org/10.1002/mbo3.1320

URI (permanent link to this page)

https://res.slu.se/id/publ/119176