Identification of fungi, especially Stachybotrys chartarum from gypsum boards, by means of PCR and sequencing of ribosomal DNA

Abstract

Gypsum boards infested by Stachybotrys chartarum are often found in built-in constructions. A PCR-based analysis method has been developed for S. chartarum using specific primers based on the Tri5 gene. Another method for detecting fungi is by species identification via sequencing of ribosomal DNA. Sequencing of ITS (Internal Transcribed Spacer) and the 5.8s rDNA is straightforward and provides a basis for species identification. The sequences were searched for by means of BLAST (Basic Local Alignment Search Tool) in the GenBank. The PCR technique will be an important step in the future both toward detecting fungal infestations at an early stage because of the ability to detect specifically the infestation without time-consuming cultivation in the laboratory and allowing reliable species identification based on sequences obtained from databases

Published in

Indoor and Built Environment
2003, volume: 12, number: 4, pages: 227-229
Publisher: SAGE PUBLICATIONS LTD

SLU Authors

• Högberg, Nils

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Land, Carl Johan

  • Department of Forest Products, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Environmental Sciences and Nature Conservation
Economics and Business
Forest Science

Publication identifier

• DOI: https://doi.org/10.1177/142032603035165

Permanent link to this page (URI)

https://res.slu.se/id/publ/1194