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Research article - Peer-reviewed, 2022

Target of Rapamycin Regulates Photosynthesis and Cell Growth in Auxenochlorella pyrenoidosa

Zhu, Tingting; Li, Linxuan; Chang, Huimin; Zhan, Jiasui; Ren, Maozhi;

Abstract

Auxenochlorella pyrenoidosa is an efficient photosynthetic microalga with autotrophic growth and reproduction, which has the advantages of rich nutrition and high protein content. Target of rapamycin (TOR) is a conserved protein kinase in eukaryotes both structurally and functionally, but little is known about the TOR signalling in Auxenochlorella pyrenoidosa. Here, we found a conserved ApTOR protein in Auxenochlorella pyrenoidosa, and the key components of TOR complex 1 (TORC1) were present, while the components RICTOR and SIN1 of the TORC2 were absent in Auxenochlorella pyrenoidosa. Drug sensitivity experiments showed that AZD8055 could effectively inhibit the growth of Auxenochlorella pyrenoidosa, whereas rapamycin, Torin1 and KU0063794 had no obvious effect on the growth of Auxenochlorella pyrenoidosa a. Transcriptome data results indicated that Auxenochlorella pyrenoidosa TOR (ApTOR) regulates various intracellular metabolism and signaling pathways in Auxenochlorella pyrenoidosa. Most genes related to chloroplast development and photosynthesis were significantly down-regulated under ApTOR inhibition by AZD8055. In addition, ApTOR was involved in regulating protein synthesis and catabolism by multiple metabolic pathways in Auxenochlorella pyrenoidosa. Importantly, the inhibition of ApTOR by AZD8055 disrupted the normal carbon and nitrogen metabolism, protein and fatty acid metabolism, and TCA cycle of Auxenochlorella pyrenoidosa cells, thus inhibiting the growth of Auxenochlorella pyrenoidosa. These RNA-seq results indicated that ApTOR plays important roles in photosynthesis, intracellular metabolism and cell growth, and provided some insights into the function of ApTOR in Auxenochlorella pyrenoidosa.

Keywords

TOR; photosynthesis; cell growth; AZD8055; Auxenochlorella pyrenoidosa

Published in

International Journal of Molecular Sciences

2022, volume: 23, number: 19, article number: 11309
Publisher: MDPI

Authors' information

Zhu, Tingting
Institute of Urban Agriculture, CAAS
Li, Linxuan
Institute of Urban Agriculture, CAAS
Li, Linxuan
Zhengzhou University
Chang, Huimin
Zhengzhou University
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Ren, Maozhi
Institute of Urban Agriculture, CAAS
Ren, Maozhi
Zhengzhou University

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.3390/ijms231911309

URI (permanent link to this page)

https://res.slu.se/id/publ/119450