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Research article - Peer-reviewed, 2022

Investigating the role of a putative endolysin-like candidate effector protein in Verticillium longisporum virulence

Rafiei, Vahideh; Najafi, Yasaman; Vélëz, Heriberto; Tzelepis, Georgios

Abstract

Verticillium is a genus of ascomycete fungi that encompasses several plant pathogenic species, and cause severe annual yield losses on many economically important crops worldwide. One of the most important species in this genus, is V. longisporum, which causes disease mainly on plants in the Brassicaceae family. Genome analysis of V. longisporum strain VL1 revealed a number of candidate effector genes that may be associated with fungal virulence. One of these candidate effector-genes encodes a putative endolysin-like protein. Endolysins are hydrolytic enzymes that are secreted by bacteriophages and recently, they have been identified in fungal genomes as well. In this study, the potential role of this gene has been investigated in V. longisporum. Our data showed that this gene was highly induced in the fungus during Brassica napus infection and its overexpression significantly increased V. longisporum virulence, indi-cating an involvement in the fungal infection process.(c) 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Keywords

Bacteriophages; Effectors; Endolysin; Soilborne pathogens; Verticillium

Published in

Biochemical and Biophysical Research Communications
2022, volume: 629, pages: 6-11
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.1016/j.bbrc.2022.08.086

URI (permanent link to this page)

https://res.slu.se/id/publ/119811