Abstract

Gene technology and editing are not only biotechnological techniques for creating new crop varieties but are also tools for researchers to discover gene functions. Field trial following laboratory experiments is an important step in order to evaluate new functions since many phenotypes, and combinations thereof, are difficult to detect in controlled environments and molecular analyses are nowadays possible to do in the field. Here we describe a standard protocol for creating new potato lines and producing seed tubers for field trials within 1 year.

Keywords

Potato; Solanum tuberosum; Field trials; Agrobacterium transformation; Seed tuber production; Micro tuber; In vitro long-term storage

Published in

Methods in Molecular Biology
2021, number: 2354, pages: 111-122
Book title: Solanum tuberosum : Methods and Protocols
ISBN: 978-1-0716-1608-6, eISBN: 978-1-0716-1609-3
Publisher: Springer

Authors' information