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Research article - Peer-reviewed, 2020

The transcriptome enables the identification of candidate genes behind medicinal value of Drumstick tree (Moringa oleifera)

Pasha, Shaik Naseer; Shafi, K. Mohamed; Joshi, Adwait G.; Meenakshi, Iyer; Harini, K.; Mahita, Jarjapu; Sajeevan, Radha Sivarajan; Karpe, Snehal D.; Ghosh, Pritha; Nitish, Sathyanarayanan; Gandhimathi, A.; Mathew, Oommen K.; Prasanna, Subramanian Hari; Malini, Manoharan; Mutt, Eshita; Naika, Mahantesha; Ravooru, Nithin; Rao, Rajas M.; Shingate, Prashant N.; Sukhwal, Anshul;
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Abstract

Moringa oleifera is a plant well-known for its nutrition value, drought resistance and medicinal properties. cDNA libraries from five different tissues (leaf, root, stem, seed and flower) of M. oleifera cultivar Bhagya were generated and sequenced. We developed a bioinformatics pipeline to assemble transcriptome, along with the previously published M. oleifera genome, to predict 17,148 gene models. Few candidate genes related to biosynthesis of secondary metabolites, vitamins and ion transporters were identified. Expressions were further confirmed by real-time quantitative PCR experiments for few promising leads. Quantitative estimation of metabolites, as well as elemental analysis, was also carried out to support our observations. Enzymes in the biosynthesis of vitamins and metabolites like quercetin and kaempferol are highly expressed in leaves, flowers and seeds. The expression of iron transporters and calcium storage proteins were observed in root and leaves. In general, leaves retain the highest amount of small molecules of interest.

Keywords

Plant transcriptome; Functional annotation; Differential expression; Metabolic pathway analysis

Published in

Genomics
2020, volume: 112, number: 1, pages: 621-628

Authors' information

Pasha, Shaik Naseer
Tata Institute of Fundamental Research (TIFR)
Shafi, K. Mohamed
Tata Institute of Fundamental Research (TIFR)
Joshi, Adwait G.
National Centre for Biological Sciences (NCBS)
Meenakshi, Iyer
Tata Institute of Fundamental Research (TIFR)
Harini, K.
National Centre for Biological Sciences (NCBS)
Mahita, Jarjapu
National Centre for Biological Sciences (NCBS)
Radha Sivarajan, Sajeevan
Tata Institute of Fundamental Research
Karpe, Snehal D.
National Centre for Biological Sciences (NCBS)
Ghosh, Pritha
National Centre for Biological Sciences (NCBS)
Nitish, Sathyanarayanan
Tata Institute of Fundamental Research (TIFR)
Gandhimathi, A.
Tata Institute of Fundamental Research (TIFR)
Mathew, Oommen K.
Tata Institute of Fundamental Research (TIFR)
Prasanna, Subramanian Hari
Tata Institute of Fundamental Research (TIFR)
Malini, Manoharan
National Centre for Biological Sciences (NCBS)
Mutt, Eshita
Tata Institute of Fundamental Research (TIFR)
Naika, Mahantesha
Tata Institute of Fundamental Research (TIFR)
Ravooru, Nithin
National Centre for Biological Sciences (NCBS)
Rao, Rajas M.
Tata Institute of Fundamental Research (TIFR)
Shingate, Prashant N.
Tata Institute of Fundamental Research (TIFR)
Sukhwal, Anshul
Tata Institute of Fundamental Research (TIFR)
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UKÄ Subject classification

Genetics

Publication Identifiers

DOI: https://doi.org/10.1016/j.ygeno.2019.04.014

URI (permanent link to this page)

https://res.slu.se/id/publ/120211