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Research article - Peer-reviewed, 2022

Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity

Shafi KM, Sajeevan RS, Kouser S, Vishnuprasad CN, Sowdhamini R

Abstract

Background
Moringa concanensis Nimmo (MC), a plant that resembles Moringa oleifera Lam. (MO), has less scientific information but has traditionally been used as a medicinal plant. Moringa species have long been known for their medicinal qualities, which include antioxidant, anti-inflammatory, anticancer, and antihyperglycemic effects. We investigated the antidiabetic potential of MC and MO species in this study by using transcriptome profiling, metabolite analysis, and in vitro assay studies.
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Results
Our transcriptome analysis revealed the expression of enzymes involved in the biosynthesis of quercetin, chlorogenic acid, and benzylamine, all of which have previously been shown to have antidiabetic activity. We compared the expression patterns of five different tissues from MC and MO and it was found that the key enzymes involved in the biosynthesis of these compounds were highly expressed in leaf tissue. The expression estimated by MC transcriptome data in different tissues was verified using RT-qPCR analysis. The amount of these compounds was further quantified in the crude leaf extract of both species and found that MC had a higher abundance of quercetin and chlorogenic acid than MO. The crude leaf extract from both MC and MO were further tested in vitro, and the results demonstrated strong inhibitory activity for α-glucosidase and DPP-IV enzymes. Our findings suggest that compounds in leaf tissue, such as quercetin, benzylamine, and chlorogenic acid, could play a significant role in this antidiabetic activity. In addition, when comparing MO plants, we found that MC had a slightly higher effect in expression, abundance, and inhibitory activity.

Conclusions
This study presents the first report of MC transcriptome data, as well as a comparison of its anti-diabetic activity to MO. Our analysis discussed the significance of leaf tissue in antidiabetic activity compared to other tissues of both species. Overall, this study not only provides transcriptome resources for Moringa species, but also sheds light on antidiabetic potential of both species.

Published in

BMC Plant Biology
2022, volume: 22, article number: 561
Publisher: Springer Science and Business Media {LLC}

Authors' information

Shafi, K. Mohamed
National Centre for Biological Sciences (NCBS)
National Centre for Biological Sciences (NCBS)
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Kouser, Sania
University of Trans-Disciplinary Health Sciences & Technology
Vishnuprasad, Chethala N
Univ Trans Disciplinary Hlth Sci and Technol TDU
Sowdhamini, Ramanathan
National Centre for Biological Sciences (NCBS)

UKÄ Subject classification

Plant Biotechnology

Publication Identifiers

DOI: https://doi.org/10.1186/s12870-022-03938-6

URI (permanent link to this page)

https://res.slu.se/id/publ/120212