Structure-function studies of GH7 cellulases, key enzymes in the global carbon cycleHaataja, Topi
Enzyme mixtures used for lignocellulosic ethanol production are most commonly derived from filamentous fungi, and enzymes from the glycoside hydrolase family 7 (GH7) constitute the most abundant components in these cocktails. In this thesis I have aimed to increase our understanding of this enzyme family, with focus on the interrelation between their structure and function.
In a study of the two model enzymes Trichoderma reesei Cel7A (TreCel7A) and Phanerochaete chrysosporium Cel7D (Pch7D), we determined factors governing the idiosyncratic behavior of these enzymes on commonly used model compounds, and by using fluorescence titration, enzyme kinetics, structure determination and molecular dynamics simulations found specific structural features connected to nonproductive binding, playing a major role in enzyme activity on these compounds.
We also determined the molecular structure of a GH7 enzyme RsSymEG1, belonging to a group of smaller GH7 endoglucanases with previously unknown structure architecture, and originating from symbiotic protozoa of wood eating lower termites. The X-ray crystal structure revealed a configuration with several key
differences to previously known GH7 structures, and will aid in modelling and engineering of enzymes in this so far little-known group of enzymes. A further look into this group of sequences, as well as other GH7 enzymes found in the termite symbiont protists, also revealed previously unknown details about the evolution of
this ancient enzyme family.
Furthermore, we explored single molecule imaging of the model enzyme
TreCel7A with novel imaging methods, providing a first proof-of-concept of using
fluorescence resonance energy transfer (FRET) for the study of inter-domain
dynamics of this enzyme, as well as total internal reflection dark-field microscopy
(TIRDFM) for imaging enzyme movement on cellulose surface at ultra-high
KeywordsCellulase; lignocellulose; GH7; glycoside hydrolase; biofuel; fungi
Published inActa Universitatis Agriculturae Sueciae
2023, number: 2023:9
ISBN: 978-91-8046-070-5, eISBN: 978-91-8046-071-2
Publisher: Swedish University of Agricultural Sciences
UKÄ Subject classification
Biochemistry and Molecular Biology
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