Skip to main content
Research article - Peer-reviewed, 2022

Transcriptional Regulation of zma-MIR528a by Action of Nitrate and Auxin in Maize

Lujan-Soto, Eduardo; Aguirre de la Cruz, Paola, I; Juarez-Gonzalez, Vasti T.; Reyes, Jose L.; de la Paz Sanchez, Maria; Dinkova, Tzvetanka D.


In recent years, miR528, a monocot-specific miRNA, has been assigned multifaceted roles during development and stress response in several plant species. However, the transcription regulation and the molecular mechanisms controlling MIR528 expression in maize are still poorly explored. Here we analyzed the zma-MIR528a promoter region and found conserved transcription factor binding sites related to diverse signaling pathways, including the nitrate (TGA1/4) and auxin (AuxRE) response networks. Accumulation of both pre-miR528a and mature miR528 was up-regulated by exogenous nitrate and auxin treatments during imbibition, germination, and maize seedling establishment. Functional promoter analyses demonstrated that TGA1/4 and AuxRE sites are required for transcriptional induction by both stimuli. Overall, our findings of the nitrogen- and auxin-induced zma-MIR528a expression through cis-regulatory elements in its promoter contribute to the knowledge of miR528 regulome.


AuxRE; miR528; promoter analysis; TGA1; Zea mays

Published in

International Journal of Molecular Sciences
2022, volume: 23, number: 24, article number: 15718
Publisher: MDPI

Authors' information

Lujan-Soto, Eduardo
Universidad Nacional Autonoma de Mexico
Aguirre de la Cruz, Paola
Universidad Nacional Autonoma de Mexico
National Autonomous University of Mexico (UNAM)
Swedish University of Agricultural Sciences, Department of Plant Biology
Reyes, Jose L.
Universidad Nacional Autonoma de Mexico
de la Paz Sanchez, Maria
Universidad Nacional Autonoma de Mexico
Dinkova, Tzvetanka D.
Universidad Nacional Autonoma de Mexico

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers


URI (permanent link to this page)