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Research article2023Peer reviewedOpen access

Protein Identification of Seminal Plasma in Bali Bull (Bos javanicus)

Iskandar, Hikmayani; Andersson, Goeran; Sonjaya, Herry; Arifiantini, Raden Iis; Said, Syahruddin; Hasbi, Hasbi; Maulana, Tulus; Baharun, Abdullah


Simple Summary In this study, in order to provide a better understanding of and new knowledge about seminal plasma protein in Bali bull (Bos javanicus), we analyzed plasma semen using LC-MS/MS. The results of the analysis were then analyzed using bioinformatics to explore the total protein, expression, and inferred function of these seminal plasma proteins of Bali bulls. We identified six proteins that have previously been associated with fertility. These studies identified seminal plasma proteins that will have potential as biomarkers of fertility in Bali bulls. The purpose of this study was to identify seminal plasma proteins in Bali bull and their potential as biomarkers of fertility. Semen was collected from 10 bulls aged 5-10 years using an artificial vagina. Fresh semen was then centrifuged (3000x g for 30 min). The supernatant was put into straws and stored in liquid nitrogen. The semen plasma protein concentration was determined using the Bradford method, and the protein was characterized using 1D-SDS-PAGE. Coomassie Brilliant Blue (CBB) was used to color the gel, and the molecular weight of the protein was determined using PM2700. A total of 94 proteins were identified in the seminal plasma of Bali bulls analyzed using LC-MS/MS (liquid chromatography-mass spectrometry). Proteins spermadhesin 1 (SPADH1), C-type natriuretic peptide (NPPC), clusterin (CLU), apoliprotein A-II (APOA2), inositol-3-phosphate synthase 1 (ISYNA1), and sulfhydryl oxidase 1 (QSOX1) were identified as important for fertility in Bos javanicus. These proteins may prove to be important biomarkers of fertility in Bali bulls. These proteins are important for reproductive function, which includes spermatozoa motility, capacitation, and acrosome reactions. This study provides new information about the protein content in seminal plasma in Bali bulls. The LC-MS/MS-based proteome approach that we applied in this study obtained 94 proteins. The identification of these seminal plasma proteins of Bali bulls and their potential as fertility biomarkers may have an impact on the success of future artificial insemination (AI).


Bali bull; seminal plasma; protein; fertility; biomarkers

Published in

2023, Volume: 13, number: 3, article number: 514
Publisher: MDPI

    UKÄ Subject classification

    Animal and Dairy Science

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