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Research article - Peer-reviewed, 2023

Physcomitrium patens PpRIC, an ancestral CRIB-domain ROP effector, inhibits auxin-induced differentiation of apical initial cells

Ntefidou, Maria; Eklund, D. Magnus; Le Bail, Aude; Schulmeister, Sylwia; Scherbel, Franziska; Brandl, Lisa; Doerfler, Wolfgang; Eichstaedt, Chantal; Bannmueller, Anna; Ljung, Karin; Kost, Benedikt


RHO guanosine triphosphatases are important eukaryotic regulators of cell differentiation and behavior. Plant ROP (RHO of plant) family members activate specific, incompletely characterized downstream signaling. The structurally simple land plant Physcomitrium patens is missing homologs of key animal and flowering plant RHO effectors but contains a single CRIB (CDC42/RAC interactive binding)-domain -contain-ing RIC (ROP-interacting CRIB-containing) protein (PpRIC). Protonemal P. patens filaments elongate based on regular division and PpROP-dependent tip growth of apical initial cells, which upon stimulation by the hor-mone auxin differentiate caulonemal characteristics. PpRIC interacts with active PpROP1, co-localizes with this protein at the plasma membrane at the tip of apical initial cells, and accumulates in the nucleus. Remark-ably, PpRIC is not required for tip growth but is targeted to the nucleus to block caulonema differentiation downstream of auxin-controlled gene expression. These observations establish functions of PpRIC in medi-ating crosstalk between ROP and auxin signaling, which contributes to the maintenance of apical initial cell identity.

Published in

Cell Reports
2023, volume: 42, number: 2, article number: 112130
Publisher: CELL PRESS

Authors' information

Ntefidou, Maria
University of Erlangen-Nuremberg
Eklund, D. Magnus
Uppsala University
Le Bail, Aude
University of Erlangen-Nuremberg
Schulmeister, Close Sylwia
University of Erlangen-Nuremberg
Scherbel, Franziska
University of Erlangen-Nuremberg
Brandl, Lisa
University of Erlangen-Nuremberg
Dörfler, Wolfgang
University of Erlangen-Nuremberg
Eichstädt, Chantal
University of Erlangen-Nuremberg
Bannmüller, Anna
University of Erlangen-Nuremberg
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Kost, Benedikt
University of Erlangen-Nuremberg

UKÄ Subject classification

Cell Biology

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