Ljung, Karin
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Research article2023Peer reviewedOpen access
Ntefidou, Maria; Eklund, D. Magnus; Le Bail, Aude; Schulmeister, Sylwia; Scherbel, Franziska; Brandl, Lisa; Doerfler, Wolfgang; Eichstaedt, Chantal; Bannmueller, Anna; Ljung, Karin; Kost, Benedikt
RHO guanosine triphosphatases are important eukaryotic regulators of cell differentiation and behavior. Plant ROP (RHO of plant) family members activate specific, incompletely characterized downstream signaling. The structurally simple land plant Physcomitrium patens is missing homologs of key animal and flowering plant RHO effectors but contains a single CRIB (CDC42/RAC interactive binding)-domain -contain-ing RIC (ROP-interacting CRIB-containing) protein (PpRIC). Protonemal P. patens filaments elongate based on regular division and PpROP-dependent tip growth of apical initial cells, which upon stimulation by the hor-mone auxin differentiate caulonemal characteristics. PpRIC interacts with active PpROP1, co-localizes with this protein at the plasma membrane at the tip of apical initial cells, and accumulates in the nucleus. Remark-ably, PpRIC is not required for tip growth but is targeted to the nucleus to block caulonema differentiation downstream of auxin-controlled gene expression. These observations establish functions of PpRIC in medi-ating crosstalk between ROP and auxin signaling, which contributes to the maintenance of apical initial cell identity.
Cell Reports
2023, Volume: 42, number: 2, article number: 112130Publisher: CELL PRESS
Cell Biology
DOI: https://doi.org/10.1016/j.celrep.2023.112130
https://res.slu.se/id/publ/121653