Skip to main content
Research article - Peer-reviewed, 2023

Stability analyses of sunflower (Helianthus annuus L.) hybrids for oleic acid and yield traits under multi location trials in Pakistan

Shah, Masood Hussain; Rauf, Saeed; Nazir, Shahid; Ortiz, Rodomiro; Naveed, Abdul; Fatima, Seerat


The development of a hybrid with high oleic acid is an important breeding goal for sunflower. High oleic acid sunflower has better cooking quality due to low oxidation and rancidity. Hence, inbred lines differing for oleic acid content were selected, alongside the development of hybrids where one or both parents exhibited high oleic acid content in edible oil, and then evaluated at various sites (i.e. with comparatively low temperature during sunflower reproductive phase at Sargodha and Faisalabad; while high temperature and low humidity at Bhawalpur and Multan) in Pakistan during spring season. Moreover, autumn season was relatively cool and high humid for sites (Faisalabad and Sargodha, Pakistan). DNA profiling of hybrids differing for oleic acid content using N1-3F/N2-1R confirmed the presence of a high oleic acid allele in the hybrids. Oleic acid content and seed yield components were increased at high temperature and low humidity to a greater extent in spring than in autumn season. Among the hybrids, one (H5) had stable high oleic acid content during the spring season with higher seed yield and kernel to seed percentage than the check cultivars (Hysun-33 and FH-331). Analysis of the combining ability of two locations revealed a relationship between mean oleic acid contents and combining ability, thereby suggesting the effectiveness of selection in developing high oleic acid inbred lines. Newly developed inbred C.112.P was a positive combiner for oleic acid at all sites except Sargodha, while restorer populations such as RH.344, RH.345 and RH.347 were positive male combiners.


combining ability; degree days; kernel to seed percentage; seed yield

Published in

Italian Journal of Agronomy
2023, Volume: 18, number: 1, article number: 2079

    SLU Authors

    Sustainable Development Goals

    SDG3 Good health and well-being
    SDG2 Zero hunger
    SDG1 No poverty

    UKÄ Subject classification

    Agricultural Science
    Genetics and Breeding

    Publication Identifiers


    Permanent link to this page (URI)