Abstract

Natural variation in the Arabidopsis thaliana/cauliflower mosaic virus pathosystem uncovers a broad spectrum of disease outcomes and identifies the ABA biosynthesis gene NCED9as a novel susceptibility factor.Viruses are intimately linked with their hosts and especially dependent on gene-for-gene interactions to establish successful infections. On the host side, defence mechanisms such as tolerance and resistance can occur within the same species, leading to differing virus accumulation in relation to symptomology and plant fitness. The identification of novel resistance genes against viruses and susceptibility factors is an important part of understanding viral patho-genesis and securing food production. The model plant Arabidopsis thaliana displays a wide symptom spectrum in response to RNA virus infections, and unbiased genome-wide association studies have proven a powerful tool to identify novel disease-genes. In this study we infected natural accessions of A. thaliana with the pararetrovirus cauliflower mosaic virus (CaMV) to study the phenotypic variations between accessions and their correlation with virus accumulation. Through genome-wide association mapping of viral accumulation differences, we identified several susceptibility factors for CaMV, the strongest of which was the abscisic acid synthesis gene NCED9. Further experiments confirmed the importance of abscisic acid homeostasis and its disruption for CaMV disease.

Keywords

Abscisic acid; Arabidopsis; cauliflower mosaic virus; genome-wide association studies; virus disease; virus tolerance

Published in

Journal of Experimental Botany
2023, Volume: 74, number: 15, pages: 4751-4764
Publisher: OXFORD UNIV PRESS

SLU Authors

• Hoffmann, Gesa

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Linnean Centre for Plant Biology

• Shukla, Aayushi

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Linnean Centre for Plant Biology

• Lòpez Gonzàlez, Silvia

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Linnean Centre for Plant Biology

• Hafrén, Anders

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Linnean Centre for Plant Biology

Associated SLU-program

SLU Plant Protection Network
AMR: Virus


Sustainable Development Goals

End hunger, achieve food security and improved nutrition and promote sustainable agriculture


UKÄ Subject classification

Botany


Publication identifier

DOI: https://doi.org/10.1093/jxb/erad204

Permanent link to this page (URI)

https://res.slu.se/id/publ/122913