Abstract

Revolutionary advances in AI and deep learning in recent years have resulted in an upsurge of papers exploring applications within the biomedical field. Within stem cell research, promising results have been reported from analyses of microscopy images to, that is, distinguish between pluripotent stem cells and differentiated cell types derived from stem cells. In this work, we investigated the possibility of using a deep learning model to predict the differentiation stage of pluripotent stem cells undergoing differentiation toward hepatocytes, based on morphological features of cell cultures. We were able to achieve close to perfect classification of images from early and late time points during differentiation, and this aligned very well with the experimental validation of cell identity and function. Our results suggest that deep learning models can distinguish between different cell morphologies, and provide alternative means of semi-automated functional characterization of stem cell cultures.

Keywords

pluripotent stem cells; cell differentiation; hepatocytes; quality control; artificial intelligence; image analysis; computer-assisted

Published in

STEM CELLS
2023, Volume: 41, number: 9, pages: 850–861
Publisher: OXFORD UNIV PRESS

SLU Authors

• Marzec Schmidt, Katarzyna

  • Department of Soil and Environment, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)


Publication identifier

DOI: https://doi.org/10.1093/stmcls/sxad049

Permanent link to this page (URI)

https://res.slu.se/id/publ/122974