Abstract

Hyaluronan (HA), a member of the GAG family of glycans, has many diverse biological functions that vary a lot depending on the length of the HA chain and its concentration. A better understanding of the structure of different-sized HA at the atomic level is therefore crucial to decipher these biological functions. NMR is a method of choice for conformational studies of biomolecules, but there are limitations due to the low natural abundance of the NMR active nuclei 13C and 15N. We describe here the metabolic labeling of HA using the bacterium Streptococcus equi subsp. Zooepidemicus and the subsequent analysis by NMR and mass spectrometry. The level of 13C and 15N isotope enrichment at each position was determined quantitatively by NMR spectroscopy and was further confirmed by high-resolution mass spectrometry analysis. This study provides a valid methodological approach that can be applied to the quantitative assessment of isotopically labeled glycans and will help improve detection capabilities and facilitate future structure-function relationship analysis of complex glycans.

Published in

Carbohydrate Research
2023, Volume: 531, article number: 108888
Publisher: ELSEVIER SCI LTD

SLU Authors

• Xue, Yan

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Gohil, Suresh

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Nestor, Gustav

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Sandström, Corine

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1016/j.carres.2023.108888

Permanent link to this page (URI)

https://res.slu.se/id/publ/123042