Ortiz Rios, Rodomiro Octavio
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Abstract
Mutation breeding based on various chemical and physical mutagens induces and disrupts non-target loci. Hence, large populations were required for visual screening, but desired plants were rare and it was a further laborious task to identify desirable mutants. Generated mutant had high defect due to non-targeted mutation, with poor agronomic performance. Mutation techniques were augmented by targeted induced local lesions in genome (TILLING) facilitating the selection of desirable germplasm. On the other hand, gene editing through CRISPR/Cas9 allows knocking down genes for site-directed mutation. This handy technique has been exploited for the modification of fatty acid profile. High oleic acid genetic stocks were obtained in a broad range of crops. Moreover, genes involved in the accumulation of undesirable seed components such as starch, polysaccharide, and flavors were knocked down to enhance seed quality, which helps to improve oil contents and reduces the anti-nutritional component.
Keywords
Gene editing; genome; induced mutation; oil contents; sunflower; >
Published in
GM Crops & Food
2023, volume: 14, number: 1, pages: 1-12
Publisher: TAYLOR AND FRANCIS AS
SLU Authors
Global goals (SDG)
SDG1 No poverty
SDG2 Zero hunger
SDG13 Climate action
UKÄ Subject classification
Plant Biotechnology
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1080/21645698.2023.2243041
Permanent link to this page (URI)
https://res.slu.se/id/publ/123055