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Review article2023Peer reviewedOpen access

Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate

Rauf, Saeed; Fatima, Seerat; Ortiz, Rodomiro


Mutation breeding based on various chemical and physical mutagens induces and disrupts non-target loci. Hence, large populations were required for visual screening, but desired plants were rare and it was a further laborious task to identify desirable mutants. Generated mutant had high defect due to non-targeted mutation, with poor agronomic performance. Mutation techniques were augmented by targeted induced local lesions in genome (TILLING) facilitating the selection of desirable germplasm. On the other hand, gene editing through CRISPR/Cas9 allows knocking down genes for site-directed mutation. This handy technique has been exploited for the modification of fatty acid profile. High oleic acid genetic stocks were obtained in a broad range of crops. Moreover, genes involved in the accumulation of undesirable seed components such as starch, polysaccharide, and flavors were knocked down to enhance seed quality, which helps to improve oil contents and reduces the anti-nutritional component.


Gene editing; genome; induced mutation; oil contents; sunflower; >

Published in

GM Crops & Food
2023, Volume: 14, number: 1, pages: 1-12

    Sustainable Development Goals

    SDG1 No poverty
    SDG2 Zero hunger
    SDG13 Climate action

    UKÄ Subject classification

    Plant Biotechnology
    Agricultural Science

    Publication identifier


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