Abstract

Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded fluorescent proteins, which are useful for understanding of plant development. To visualize suberin lamellae by fluorescent microscopy, we improved a histological staining procedure with the dyes Fluorol Yellow 088 and aniline blue. In the complex plant organs such as roots, suberin lamellae are deposited deep within the root on the endodermal cell wall. Our procedure yields reliable and detailed images that can be used to determine the suberin pattern in root cells. The main advantage of this protocol is its efficiency, the detailed visualization of suberin localization it generates in the root, and the possibility of returning to the confocal images to analyze and re-evaluate data if necessary.

Keywords

Suberin; Fluorol Yellow; Root; Confocal microscopy

Published in

Bio-protocol
2021, Volume: 11, number: 3, article number: e3904
Publisher: BIO-PROTOCOL

SLU Authors

• Marhavy, Peter

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany
Cell Biology


Publication identifier

DOI: https://doi.org/10.21769/BioProtoc.3904

Permanent link to this page (URI)

https://res.slu.se/id/publ/123205