Abstract

Soil organic carbon (SOC) originates from a complex mixture of organic materials, and to better understand its role in soil functions, one must characterise its chemical composition. However, current methods, such as solid-state C-13 nuclear magnetic resonance (NMR) spectroscopy, are time-consuming and expensive. Diffuse reflectance spectroscopy in the visible, near infrared and mid-infrared regions (vis-NIR: 350-2500 nm; mid-IR: 4000-400 cm(-1)) can also be used to characterise SOC chemistry; however, it is difficult to know the frequencies where the information occurs. Thus, we correlated the C functional groups from the C-13 NMR to the frequencies in the vis-NIR and mid-IR spectra using two methods: (1) 2-dimensional correlations of C-13 NMR spectra and the diffuse reflectance spectra, and (2) modelling the NMR functional C groups with the reflectance spectra using support vector machines (SVM) (validated using 5 times repeated 10-fold cross-validation). For the study, we used 99 mineral soils from the agricultural regions of Sweden. The results show clear correlations between organic functional C groups measured with NMR and specific frequencies in the vis-NIR and mid-IR spectra. While the 2D correlations showed general relationships (mainly related to the total SOC content), analysing the importance of the wavelengths in the SVM models revealed more detail. Generally, models using mid-IR spectra produced slightly better estimates than the vis-NIR. The best estimates were for the alkyl C group (R-2 = 0.83 and 0.85, vis-NIR and mid-IR, respectively), and the O/N-alkyl C group was the most difficult to estimate (R-2 = 0.34 and 0.38, vis-NIR and mid-IR, respectively). Combining C-13 NMR with the cost-effective diffuse reflectance methods could potentially increase the number of measured samples and improve the spatial and temporal characterisation of SOC. However, more studies with a wider range of soil types and land management systems are needed to further evaluate the conditions under which these methods could be used. Highlights Diffuse reflectance spectroscopy was used to characterise and model SOC functional chemistry. NMR derived C functional groups could be modelled with vis-NIR and mid-IR diffuse reflectance spectra. The methods allow for characterisation of SOC chemical composition on whole mineral soil samples. The approach can improve the spatial and temporal characterisation of SOC composition.

Keywords

C-13 NMR; C functional groups; C turnover; mid-IR spectroscopy; NIR spectroscopy; soil organic matter composition; soil organic matter quality

Published in

European Journal of Soil Science
2022, Volume: 73, number: 4, article number: e13263
Publisher: WILEY

SLU Authors

• Wetterlind, Johanna

  • Department of Soil and Environment, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Soil Science


Publication identifier

DOI: https://doi.org/10.1111/ejss.13263

Permanent link to this page (URI)

https://res.slu.se/id/publ/123374