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Report, 1967

The homozygous and heterozygous effects of an aurea mutation on plastid development in spruce (Picea abies (L.) Karst.)

Walles, Björn


A11 electron niicroscopical investigation has been made of plastid developnienti n spruce plants which are homozygous or heterozygous for a semi-dominant aurea mutation. The cotyledons of normal spruce seedlings synthesise chlorophyll and develop chloroplasts both in the dark and in the light. The chloroplasts in dark-grown seedlings have grana and severa1 prolamellar bodies. No chlorophyll was detected in acetone extracts of light-grown or dark-grown seedlings which were homozygous for the aurea factor. In these yellow lethal seedlings,plastid morphogenesis is blocked prior to the formation of prolamellar bodies. In the yellon-green heterozygous aurea seedlings, clorophyll synthesis is reduced; when grown in the dark they have a clorophyll content that is only 50 per cent of that in corresponding wild type individuals. The chloroplasts of these dark-grown aurea seedlings possess several prolamellar bodies and a poorly differentiated lamellar system, in which the grana usually contain only two or three discs. Aurea plants develop in the light an abnormal lamellar system with grana which appear sickle-shaped in cross-section. The yellon-green needles possess the ability of spontaneous greening. The chloroplasts in older green needles of the mutant are normalised and indistinguishable from wild type chloroplasts.


homozygous effects; heterozygous effects; Aurea mutation; Picea abies; plastid development; seedlings

Published in

Studia Forestalia Suecica
Publisher: Skogshögskolan

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