Abstract

The aim of the present study was to test a rapid, robust flow cytometric technique for the detection of sperm head abnormalities of domestic bulls and stallions. The so-called PulSA approach detects the pulse profiles of propidium-iodide labelled spermatozoa. In the first experiment, species-specific threshold values were established on sperm samples that were tested for sperm head abnormalities with a classic visual morphology analysis. In the second experiment, serial mixtures of bull and stallion spermatozoa mimicking different percentages of sperm head abnormalities were analysed. Non-metric multidimensional scaling showed a clear separation between the normal and mixed samples. The PulSA approach may be a useful tool in identifying sub- or infertile breeding males as well as in studying the evolutionary aspects of sperm morphology and morphometry.

Keywords

abnormalities; bull; PulSA; sperm quality; stallion

Published in

Reproduction in Domestic Animals
2023, Volume: 58, number: 11, pages: 1569-1575
Publisher: WILEY

SLU Authors

• Johannisson, Anders

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Uppsala University

UKÄ Subject classification

Clinical Science
Animal and Dairy Science


Publication identifier

DOI: https://doi.org/10.1111/rda.14472

Permanent link to this page (URI)

https://res.slu.se/id/publ/126499