Abstract

The enteropathogenic Yersinia genus is commonly detected in wildlife including wild boars. Difficulties in its cultivation may hamper subsequent epidemiological studies and outbreak investigations. Multiple-locus variable-number tandem repeat analysis (MLVA) of Yersinia (Y.) enterocolitica and Y. pseudotuberculosis has proven useful in source attribution and epidemiological studies but has hitherto relied on the analysis of isolates. In the present study, MLVA profiles generated from 254 isolates of Y. enterocolitica indicated similarities between human, pig and rodent isolates. Further, MLVA analyses of 13 Y. pseudotuberculosis pure-cultured isolates were compared to MLVA analyses performed directly on the 14 PCR-positive enrichment broths from which the isolates originated, which showed matching MLVA profiles. This indicates that MLVA analysis performed directly on enrichment broths could be a useful method for molecular epidemiological investigations. In addition, 10 out of 32 samples of wild boar minced meat obtained from private hunters and from approved wild-game-handling establishments were PCR-positive for the presence of Y. enterocolitica and may indicate a risk for public health.

Keywords

Y. enterocolitica; Y. pseudotuberculosis; multiple-locus variable-number tandem repeat analysis (MLVA)

Published in

Animals
2023, Volume: 13, article number: 3055
Publisher: MDPI

SLU Authors

• Sannö, Axel

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Jacobson, Magdalena

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Clinical Science


Publication identifier

DOI: https://doi.org/10.3390/ani13193055

Permanent link to this page (URI)

https://res.slu.se/id/publ/126807