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Research article - Peer-reviewed, 2023

Genomic, phenotypic, and clinical safety of Limosilactobacillus reuteri ATCC PTA 4659

Sendelius, Malin; Axelsson, Jakob; Liu, Peidi; Roos, Stefan

Abstract

Evaluating the safety of probiotic microorganisms is an important part of the development of probiotic products. In this study, we have performed a systematic safety assessment of Limosilactobacillus reuteri American Type Culture Collection (ATCC) PTA 4659 based on genome analysis, antibiotic susceptibility testing, phenotypic characterization, and a human clinical safety study. Genome sequence analysis showed that the strain is free from virulence and antibiotic resistance genes. Connected to this, phenotypic characterization showed that the strain is susceptible to the main classes of antibiotics. Limosilactobacillus reuteri ATCC PTA 4659 was shown to produce histamine, which has previously been described as an anti-inflammatory mediator produced by certain L. reuteri strains. However, the amount of histamine, a biogenic amine, poses no safety concern of a potential product. The strain was investigated in a human clinical safety study and was shown to survive passage through the gastrointestinal tract, both when administered at high [1 x 1011 colony-forming units (CFU)/day] and low doses (1 x 109 CFU/day). The clinical safety evaluation showed that the doses administered are safe for human consumption. Furthermore, carbohydrate utilization, mucus adhesion, and tolerance to acid and bile were studied. It was shown that L. reuteri ATCC PTA 4659 has a very high adhesion to mucus and tolerance to both gastric pH and bile, all potentially important properties for a probiotic strain. Altogether, this study has demonstrated that Limosilactobacillus reuteri ATCC PTA 4659 is safe for human consumption and along with its phenotypic characteristics and previously described anti-inflammatory effects, makes it a promising strain for future probiotic development. NCT01033539Graphical Abstract This is a systematic safety assessment of Limosilactobacillus reuteri ATCC PTA 4659 through genome analysis, phenotypic characterization, and a human clinical safety study.

Keywords

Probiotics; Limosilactobacillus reuteri; L. reuteri ATCC PTA 4659; Safety assessment

Published in

Journal of Industrial Microbiology and Biotechnology
2023, Volume: 50, number: 1, article number: kuad041
Publisher: OXFORD UNIV PRESS

    UKÄ Subject classification

    Microbiology

    Publication identifier

    DOI: https://doi.org/10.1093/jimb/kuad041

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/127651