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Research article2024Peer reviewedOpen access

Melatonin imparts tolerance to combined drought and high-temperature stresses in tomato through osmotic adjustment and ABA accumulation

Mumithrakamatchi, Annadurai K.; Alagarswamy, Senthil; Anitha, Kuppusamy; Djanaguiraman, Maduraimuthu; Kalarani, M. Karuppasami; Swarnapriya, Ramakrishnan; Marimuthu, Subramanian; Vellaikumar, Sampathrajan; Kanagarajan, Selvaraju

Abstract

In recent years, environmental stresses viz., drought and high-temperature negatively impacts the tomato growth, yield and quality. The effects of combined drought and high-temperature (HT) stresses during the flowering stage were investigated. The main objective was to assess the effects of foliar spray of melatonin under both individual and combined drought and HT stresses at the flowering stage. Drought stress was imposed by withholding irrigation, whereas HT stress was imposed by exposing the plants to an ambient temperature (AT)+5 degrees C temperature. The drought+HT stress was imposed by exposing the plants to drought first, followed by exposure to AT+5 degrees C temperature. The duration of individual and combined drought or HT stress was 10 days. The results showed that drought+HT stress had a significant negative effect compared with individual drought or HT stress alone. However, spraying 100 mu M melatonin on the plants challenged with individual or combined drought and HT stress showed a significant increase in total chlorophyll content [drought: 16%, HT: 14%, and drought+HT: 11%], Fv/Fm [drought: 16%, HT: 15%, and drought+HT: 13%], relative water content [drought: 10%, HT: 2%, and drought+HT: 8%], and proline [drought: 26%, HT: 17%, and drought+HT: 14%] compared with their respective stress control. Additionally, melatonin positively influenced the stomatal and trichome characteristics compared with stress control plants. Also, the osmotic adjustment was found to be significantly increased in the melatonin-sprayed plants, which, in turn, resulted in an increased number of fruits, fruit set percentage, and fruit yield. Moreover, melatonin spray also enhanced the quality of fruits through increased lycopene content, carotenoid content, titratable acidity, and ascorbic acid content, compared with the stress control. Overall, this study highlights the usefulness of melatonin in effectively mitigating the negative effects of drought, HT, and drought+HT stress, thus leading to an increased drought and HT stress tolerance in tomato.

Keywords

melatonin; drought; high-temperature; stomata; proline; abscisic acid; trichomes; water use efficiency

Published in

Frontiers in Plant Science
2024, Volume: 15, article number: 1382914Publisher: FRONTIERS MEDIA SA

    UKÄ Subject classification

    Botany

    Publication identifier

    DOI: https://doi.org/10.3389/fpls.2024.1382914

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/129360