Quantitative regeneration: Skoog and Miller revisited

Abstract

In 1957, Skoog and Miller published their seminal work on the effects of hormones upon plant growth. By varying the concentrations of auxin and cytokinin, they observed dramatic differences in shoot and root growth from tobacco stem cultures. Their finding that quantitative differences in hormone concentrations could dramatically alter the fate of developing organs provided a foundation for understanding organ formation and tissue regeneration. Their in vitro assays established plant propagation techniques that were critical for regenerating transgenic plants. Here, I discuss their original paper, what led to their findings and its impact on our understanding of hormone interactions, how plants regenerate and in vitro tissue culture techniques.

Keywords

auxin; cytokinin; in vitro propagation; organ patterning; regeneration

Published in

Quantitative Plant Biology
2023, Volume: 4, article number: e10Publisher: Cambridge University Press

SLU Authors

• Melnyk, Charles

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany


Publication identifier

DOI: https://doi.org/10.1017/qpb.2023.9

Permanent link to this page (URI)

https://res.slu.se/id/publ/129475