Designing PCR Primers for the Amplification-Refractory Mutation System

Abstract

The recent development in genetic research indicates that there exists intraspecific genetic variability in many organism groups. These variations, which result in a variety of genotypes and phenotypes within a population, are called polymorphism. Mutations in different ways can alter the organism’s phenotype and affect its fitness, for example, by altering disease susceptibility or resistance. Therefore, the detection of point mutations in different genes of a population is of particular importance. The amplification-refractory mutation system technique is a PCR-based method to detect single nucleotide polymorphisms in the genome. The high repeatability, low cost, high accessibility, and no need for sophisticated technology are the main advantages of the ARMS-PCR technique, compared with other available methods such as PCR-RFLP. This chapter describes the design and analysis method of primers for the ARMS-PCR technique.

Keywords

ARMS-PCR; Molecular biology; Primer design

Published in

Methods in Molecular Biology
2022, volume: 2392, number: 2392, pages: 93-99
Title: PCR Primer Design
Publisher: Humana Press Inc.

SLU Authors

• Bahram, Mohammad

  • Department of Ecology, Swedish University of Agricultural Sciences
    
  • University of Tartu

UKÄ Subject classification

Biochemistry and Molecular Biology
Genetics and Genomics

Publication identifier

• DOI: https://doi.org/10.1007/978-1-0716-1799-1_7
• ISBN: 978-1-0716-1798-4
• eISBN: 978-1-0716-1799-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/129672