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Book chapter2022Peer reviewed

A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents

Macan, G.P.F.; Khalil, S.; Kalyandurg, P.B.; Pareek, N.; Vetukuri, R.R.


The detached leaf assay (DLA) is a nondestructive method for evaluating interactions between plants and disease-causing agents that allows quick characterization of potential pathogens’ infectivity and plants’ resistance to them. Here we show its utility for also assessing potential biological control agents (BCAs), by demonstrating its applicability for screening potential BCAs for the strawberry (Fragaria × ananassa) pathogen Phytophtora cactorum.


Antagonist; Biological control agents (BCA); Detached leaf assay; Phytophtora cactorum; Plant disease; Screening

Published in

Methods in Molecular Biology
2022, Volume: 2536, number: 2536, pages: 449-458 Title: Plant Pathology : Method and Protocols
ISBN: 978-1-0716-2516-3, eISBN: 978-1-0716-2517-0
Publisher: Springer