A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli

Abstract

Reactive oxygen species (ROS) are important signaling agents in plants and animals. They are involved in diverse processes, including activation of immune responses to pathogen infection. Biphasic detection of ROS in response to pathogen perception is becoming more popular even in important crops like potato as means of screening different germ plasms and mutants generated by for example CRISPR-Cas9 as well as identifying signaling pathways. Here we describe a detailed protocol for quantifying ROS bursts induced in potato leaf discs in response to a bacterial elicitor and Phytophthora infestans.

Keywords

flg22; Immunity; Luminol; Oxidative burst; Potato; Reactive oxygen species; ROS

Published in

Methods in Molecular Biology
2022, Volume: 2536, pages: 395-402 Title: Plant Pathology : Method and Protocols
ISBN: 978-1-0716-2516-3, eISBN: 978-1-0716-2517-0Publisher: Springer

SLU Authors

• Zahid, Muhammad Awais

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Vetukuri, Ramesh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Andreasson, Erik

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany
Microbiology


Publication identifier

DOI: https://doi.org/10.1007/978-1-0716-2517-0_22

Permanent link to this page (URI)

https://res.slu.se/id/publ/129719