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Book chapter2022Peer reviewed

Spray-Induced Gene Silencing to Study Gene Function in Phytophthora

Sundararajan, P.; Kalyandurg, P.B.; Liu, Q.; Chawade, A.; Whisson, S.C.; Vetukuri, R.R.


RNA interference (RNAi) is a conserved cellular defense mechanism mediated by double-stranded RNA (dsRNA) that can regulate gene expression through targeted destruction of mRNAs (messenger RNAs). Recent studies have shown that spraying dsRNAs or small RNAs (sRNAs) that target essential genes of pathogens on plant surfaces can confer protection against pests and pathogens. Also called spray-induced gene silencing (SIGS), this strategy can be used for disease control and for transient gene silencing to study the function of genes in plant−pathogen interactions. Furthermore, as sRNAs can move locally, systemically, and cross-kingdom during plant−microbe interactions, SIGS allows quick detection and characterization of gene functions in pathogens and plants.


Gene silencing; Oomycetes; Potato; RNAi; SIGS

Published in

Methods in Molecular Biology
2022, Volume: 2536, number: 2536, pages: 459-474 Title: Plant Pathology : Method and Protocols
ISBN: 978-1-0716-2516-3, eISBN: 978-1-0716-2517-0
Publisher: Springer