Abstract

Membrane trafficking is central to cell plate construction during plant cytokinesis. Studies on cell plate formation can provide answers to basic biology questions including molecular mechanisms of membrane trafficking, tissue patterning, and cytoskeletal dynamics. Consequently, a detailed understanding of cytokinesis depends on the characterization of molecules that function in the formation, transport, targeting, and fusion of membrane vesicles and delivery of proteins to the developing and maturing plate. This chapter describes a pipeline based on fluorescence recovery after photobleaching (FRAP) to measure and analyze turnover of peripheral or transmembrane proteins on the cell plate. The approach described here can also be applied in other biological contexts.

Keywords

Cell plate; Cytokinesis; Fluorescence recovery; FRAP; Syntaxins

Published in

Methods in Molecular Biology
2022, Volume: 2382, number: 2382, pages: 233-243 Title: Plant Cell Division : Methods and Protocols
ISBN: 978-1-0716-1743-4, eISBN: 978-1-0716-1744-1
Publisher: Springer

SLU Authors

• Moschou, Panagiotis Nikolaou

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Foundation for Research and Technology - Hellas (FORTH)
  • University of Crete

UKÄ Subject classification

Cell Biology


Publication identifier

DOI: https://doi.org/10.1007/978-1-0716-1744-1_14

Permanent link to this page (URI)

https://res.slu.se/id/publ/129764