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Research article2024Peer reviewedOpen access

Pathogen group-specific risk factors for intramammary infection in water buffalo

Singha, Shuvo; Koop, Gerrit; Rahman, Md. Mizanur; Ceciliani, Fabrizio; Addis, Maria Filippa; Howlader, Md. Matiar Rahman; Hossain, Mohammed Kawser; Piccinini, Renata; Locatelli, Clara; Persson, Ylva; Bronzo, Valerio

Abstract

A cross-sectional study was conducted to estimate the prevalence of intramammary infection (IMI) associated bacteria and to identify risk factors for pathogen group-specific IMI in water buffalo in Bangladesh. A California Mastitis Test (CMT) and bacteriological cultures were performed on 1,374 quarter milk samples collected from 763 water buffalo from 244 buffalo farms in nine districts in Bangladesh. Quarter, buffalo, and farm-related data were obtained through questionnaires and visual observations. A total of 618 quarter samples were found to be culture positive. Non-aureus staphylococci were the predominant IMI-associated bacterial species, and Staphylococcus (S.) chromogenes, S. hyicus, and S. epidermidis were the most common bacteria found. The proportion of non-aureus staphylococci or Mammaliicoccus sciuri (NASM), S. aureus, and other bacterial species identified in the buffalo quarter samples varied between buffalo farms. Therefore, different management practices, buffalo breeding factors, and nutrition were considered and further analyzed when estimating the IMI odds ratio (OR). The odds of IMI by any pathogen (OR: 1.8) or by NASM (OR: 2.2) was high in buffalo herds with poor milking hygiene. Poor cleanliness of the hind quarters had a high odds of IMI caused by any pathogen (OR: 2.0) or NASM (OR: 1.9). Twice daily milking (OR: 3.1) and farms with buffalo purchased from another herd (OR: 2.0) were associated with IMI by any pathogen. Asymmetrical udders were associated with IMI-caused by any bacteria (OR: 1.7). A poor body condition score showed higher odds of IMI by any pathogen (OR: 1.4) or by NASM (OR: 1.7). This study shows that the prevalence of IMI in water buffalo was high and varied between farms. In accordance with the literature, our data highlight that IMI can be partly controlled through better farm management, primarily by improving hygiene, milking management, breeding, and nutrition.

Published in

PLoS ONE
2024, Volume: 19, number: 4, article number: e0299929
Publisher: PUBLIC LIBRARY SCIENCE

    UKÄ Subject classification

    Pathobiology
    Animal and Dairy Science

    Publication identifier

    DOI: https://doi.org/10.1371/journal.pone.0299929

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/130842