Cortes, Andres
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Corporación colombiana de investigación agropecuaria (AGROSAVIA)
- National University of Colombia at Medellín
Research article2024Peer reviewedOpen access
Bolanos-Rojas, Ana C.; Londono-Caicedo, Jorge M.; Cortes, Andres J.; Motato-Vasquez, Viviana
Phellinotus (Polyporales) is a common genus of wood-decay fungi in tropical and subtropical areas, endemic to the Seasonally Dry Tropical Forest (SDTF) biome. However, Phellinotus diversity remains unexplored, despite being a major threat to living trees. Therefore, this study is aimed at confirming and characterizing through morphological and molecular data the first isolates of Phellinotus teixeirae in Pithecellobium dulce (Fabaceae) trees (locally referred to as 'Chiminango') from the endangered Colombian SDTF biome. Fifteen fungal specimens were recovered from living P. dulce trees, in the urban area and at the Universidad del Valle campus, and classified as P. teixeirae based on taxonomical descriptors. Phylogenetic relationships were inferred from a four-loci dataset (ribosomal and gene-coding regions), including 82 taxa covering 3991 nucleotide positions. The analysis recovered seven highly supported (>90% bootstrapping) monophyletic taxa of the 'Phellinotus Clade', and confirmed the new distribution range of P. teixeirae (100% bootstrap support), which extends approx. 1000 km north in the Neotropics. Hierarchical stratified Analysis of MOlecular VAriance (AMOVA) provided a clear genetic distinction between species (70% of variation, p-value = 0.001) and low differentiation among country of origin within species (11%, p-value = 0.044). Discriminant Analysis for Principal Components (DAPC) indicated complex clustering including closely related species, probably a signal of recent radiation and weak species boundaries. Median-joining haplotype network analysis identified unique haplotypes, which may correlate with new host colonization and population expansion (Tajima's D <= -0.5). In conclusion, this study provides the first assessment of the genetic diversity of P. teixeirae in a novel geography (SDTP) and host tree (P. dulce). However, increasing the number of isolates remains critical to understand further the genus' distribution patterns and drivers of genetic diversity.
genetic diversity; forest pathogens; fungi; Hymenochaetaceae; molecular phylogeny; Seasonally Dry Tropical Forest (SDTP); Pithecellobium dulce (Fabaceae); 'Chiminango' tree; population expansion
Forests
2024, Volume: 15, number: 6, article number: 1008Publisher: MDPI
Forest Science
DOI: https://doi.org/10.3390/f15061008
https://res.slu.se/id/publ/131239